Antibodies Tested for Tissue-based CyCIF (Full table, May 2018, Lin, et al. eLife 2018)
You can navigate through, search, and sort (by clicking the table column heading) the following table as well as download a spreadsheet copy.
This work is made available under a Creative Commons Attribution-ShareAlike 4.0 International (CC BY-SA 4.0) License
Antibodies have been tested for a mimum level of perfomance as descrbed in footnotes below; this should not be assumed to suggest “validation” of the antibody
Search Antibodies
| Antibody Name | Target Protein | Performance | Vendor | Catalog no. | Clone | Fluorophore | Research Resource Identifier | FILTER SET | Note | Link |
|---|---|---|---|---|---|---|---|---|---|---|
| Bax-488 | Bax | *** | BioLegend | 633603 | 2D2 | Alexa Fluor 488 | AB_2562171 | 488/FITC | link | |
| CD11b-488 | CD11b | *** | Abcam | AB204271 | EPR1344 | Alexa Fluor 488 | 488/FITC | link | ||
| CD4-488 | CD4 | *** | R&D Systems | FAB8165G | Polyclonal | Alexa Fluor 488 | 488/FITC | link | ||
| CD8a-488 | CD8 | *** | eBioscience | 53-0008-80 | AMC908 | Alexa Fluor 488 | AB_2574412 | 488/FITC | link | |
| cJUN-488 | cJUN | *** | Abcam | AB193780 | E254 | Alexa Fluor 488 | 488/FITC | link | ||
| CK18-488 | Cytokeratin 18 | *** | eBioscience | 53-9815-80 | LDK18 | Alexa Fluor 488 | AB_2574480 | 488/FITC | link | |
| CK8-FITC | Cytokeratin 8 | *** | eBioscience | 11-9938-80 | LP3K | FITC | AB_10548518 | 488/FITC | link | |
| CycD1-488 | CycD1 | *** | Abcam | AB190194 | EPR2241 | Alexa Fluor 488 | 488/FITC | link | ||
| Ecad-488 | E-Cadherin | *** | CST | 3199 | 24E10 | Alexa Fluor 488 | AB_10691457 | 488/FITC | link | |
| EGFR-488 | EGFR | *** | CST | 5616 | D38B1 | Alexa Fluor 488 | AB_10691853 | 488/FITC | link | |
| EpCAM-488 | EpCAM | *** | CST | 5198 | VU1D9 | Alexa Fluor 488 | AB_10692105 | 488/FITC | link | |
| HES1-488 | HES1 | *** | Abcam | AB196328 | EPR4226 | Alexa Fluor 488 | 488/FITC | link | ||
| Ki67-488 | Ki67 | *** | CST | 11882 | D3B5 | Alexa Fluor 488 | AB_2687824 | 488/FITC | link | |
| LaminA/C-488 | Lamin A/C | *** | CST | 8617 | 4C11 | Alexa Fluor 488 | AB_10997529 | 488/FITC | link | |
| LaminB1-488 | Lamin B1 | *** | Abcam | AB194106 | EPR8985(B) | Alexa Fluor 488 | 488/FITC | link | ||
| mCD3E-FITC | ms_CD3E | *** | BioLegend | 100306 | 145-2C11 | FITC | AB_312671 | 488/FITC | link | |
| mCD4-488 | ms_CD4 | *** | BioLegend | 100532 | RM4-5 | Alexa Fluor 488 | AB_493373 | 488/FITC | link | |
| MET-488 | c-MET | *** | CST | 8494 | D1C2 | Alexa Fluor 488 | AB_10999405 | 488/FITC | link | |
| mF4/80-488 | ms_F4/80 | *** | BioLegend | 123120 | BM8 | Alexa Fluor 488 | AB_893479 | 488/FITC | link | |
| MITF-488 | MITF | *** | Abcam | AB201675 | D5 | Alexa Fluor 488 | 488/FITC | link | ||
| Ncad-488 | N-Cadherin | *** | BioLegend | 350809 | 8C11 | Alexa Fluor 488 | AB_11218797 | 488/FITC | link | |
| p53-488 | p53 | *** | CST | 5429 | 7F5 | Alexa Fluor 488 | AB_10695458 | 488/FITC | link | |
| PCNA-488 | PCNA | *** | CST | 8580 | PC10 | Alexa Fluor 488 | AB_11178664 | 488/FITC | link | |
| PD1-488 | PD1 | *** | CST | 15131 | D3W4U | Alexa Fluor 488 | 488/FITC | Discontinued | link | |
| PDI-488 | PDI | *** | CST | 5051 | C81H6 | Alexa Fluor 488 | AB_10950503 | 488/FITC | link | |
| pERK-488 | pERK(T202/Y204) | *** | CST | 4344 | D13.14.4E | Alexa Fluor 488 | AB_10695876 | 488/FITC | Discontinued | link |
| pNDG1-488 | pNDG1(T346) | *** | CST | 6992 | D98G11 | Alexa Fluor 488 | AB_10827648 | 488/FITC | link | |
| POL2A-488 | POL2A | *** | Novus Biologicals | NB200-598AF488 | 4H8 | Alexa Fluor 488 | AB_2167465 | 488/FITC | link | |
| pS6(S240/244)-488 | pS6(240/244) | *** | CST | 5018 | D68F8 | Alexa Fluor 488 | AB_10695861 | 488/FITC | link | |
| S100a-488 | S100alpha | *** | Abcam | AB207367 | EPR5251 | Alexa Fluor 488 | 488/FITC | link | ||
| SQSTM1-488 | SQSTM1/p62 | *** | CST | 8833 | D1D9E3 | Alexa Fluor 488 | 488/FITC | Discontinued | link | |
| STAT3-488 | STAT3 | *** | CST | 14047 | B3Z2G | Alexa Fluor 488 | 488/FITC | link | ||
| Survivin-488 | Survivin | *** | CST | 2810 | 71G4B7 | Alexa Fluor 488 | AB_10691462 | 488/FITC | link | |
| Catenin-488 | β-Catenin | *** | CST | 2849 | L54E2 | Alexa Fluor 488 | AB_10693296 | 488/FITC | link | |
| Actin-555 | Actin | *** | CST | 8046 | 13E5 | Alexa Fluor 555 | AB_11179208 | 555/Cy3 | link | |
| CD11c-570 | CD11c | *** | eBioscience | 41-9761-80 | 118/A5 | eFluor 570 | AB_2573632 | 555/Cy3 | link | |
| CD3D-555 | CD3D | *** | Abcam | AB208514 | EP4426 | Alexa Fluor 555 | 555/Cy3 | link | ||
| CD4-570 | CD4 | *** | eBioscience | 41-2444-80 | N1UG0 | eFluor 570 | AB_2573601 | 555/Cy3 | link | |
| CD45-PE | CD45 | *** | R&D Systems | FAB1430P-100 | 2D1 | PE | AB_2237898 | 555/Cy3 | link | |
| CK7-555 | Cytokeratin 7 | *** | Abcam | AB209601 | EPR17078 | Alexa Fluor 555 | 555/Cy3 | link | ||
| cMYC-555 | cMYC | *** | Abcam | AB201780 | Y69 | Alexa Fluor 555 | 555/Cy3 | link | ||
| E2F1-555 | E2F1 | *** | Abcam | AB208078 | EPR3818(3) | Alexa Fluor 555 | 555/Cy3 | link | ||
| Ecad-555 | E-Cadherin | *** | CST | 4295 | 24E10 | Alexa Fluor 555 | 555/Cy3 | Discontinued | link | |
| EpCAM-PE | EpCAM | *** | BioLegend | 324205 | 9C4 | PE | AB_756079 | 555/Cy3 | link | |
| FOXO1a-555 | FOXO1a | *** | Abcam | AB207244 | EP927Y | Alexa Fluor 555 | 555/Cy3 | link | ||
| FOXP3-570 | FOXP3 | *** | eBioscience | 41-4777-80 | 236A/E7 | eFluor 570 | AB_2573608 | 555/Cy3 | link | |
| GFAP-570 | GFAP | *** | eBioscience | 41-9892-80 | GA5 | eFluor 570 | AB_2573655 | 555/Cy3 | link | |
| HSP90-PE | HSP90b | *** | Abcam | AB115641 | Polyclonal | PE | AB_10936222 | 555/Cy3 | link | |
| KAP1-594 | KAP1 | *** | BioLegend | 619304 | 20A1 | Alexa Fluor 594 | AB_2563298 | 555/Cy3 | Hard to inactivate | link |
| Keratin-555 | pan-Keratin | *** | CST | 3478 | C11 | Alexa Fluor 555 | AB_10829040 | 555/Cy3 | link | |
| Keratin-570 | pan-Keratin | *** | eBioscience | 41-9003-80 | AE1/AE3 | eFluor 570 | AB_11217482 | 555/Cy3 | link | |
| Ki67-570 | Ki67 | *** | eBioscience | 41-5699-80 | 20Raj1 | eFluor 570 | AB_11220088 | 555/Cy3 | link | |
| LC3-555 | LC3 | *** | CST | 13173 | D3U4C | Alexa Fluor 555 | 555/Cy3 | link | ||
| MAP2-570 | MAP2 | *** | eBioscience | 41-9763-80 | AP20 | eFluor 570 | AB_2573634 | 555/Cy3 | link | |
| pAUR-555 | pAUR1/2/3(T288/T232/T198) | *** | CST | 13464 | D13A11 | Alexa Fluor 555 | 555/Cy3 | link | ||
| pCHK2-PE | pChk2(T68) | *** | CST | 12812 | C13C1 | PE | 555/Cy3 | link | ||
| PDL1-555 | PD-L1/CD274 | *** | Abcam | AB213358 | 28-8 | Alexa Fluor 555 | 555/Cy3 | link | ||
| pH3-555 | pH3(S10) | *** | CST | 3475 | D2C8 | Alexa Fluor 555 | AB_10694639 | 555/Cy3 | link | |
| pRB-555 | pRB(S807/811) | *** | CST | 8957 | D20B12 | Alexa Fluor 555 | 555/Cy3 | link | ||
| pS6(235/236)-555 | pS6(235/236) | *** | CST | 3985 | D57.2.2E | Alexa Fluor 555 | AB_10693792 | 555/Cy3 | Discontinued | link |
| pSRC-PE | pSRC(Y418) | *** | eBioscience | 12-9034-41 | SC1T2M3 | PE | AB_2572680 | 555/Cy3 | link | |
| S6-555 | S6 | *** | CST | 6989 | 54D2 | Alexa Fluor 555 | AB_10828226 | 555/Cy3 | link | |
| SQSTM1-555 | SQSTM1/p62 | *** | Abcam | AB203430 | EPR4844 | Alexa Fluor 555 | 555/Cy3 | link | ||
| VEGFR2-555 | VEGFR2 | *** | CST | 12872 | D5B1 | Alexa Fluor 555 | 555/Cy3 | Discontinued | link | |
| VEGFR2-PE | VEGFR2 | *** | CST | 12634 | D5B1 | PE | 555/Cy3 | link | ||
| Vimentin-555 | Vimentin | *** | CST | 9855 | D21H3 | Alexa Fluor 555 | AB_10859896 | 555/Cy3 | link | |
| Vinculin-570 | Vinculin | *** | eBioscience | 41-9777-80 | 7F9 | eFluor 570 | AB_2573646 | 555/Cy3 | link | |
| gH2ax-PE | gH2ax | *** | BioLegend | 613412 | 2F3 | PE | AB_2616871 | 555/Cy5 | link | |
| AKT-647 | AKT | *** | CST | 5186 | C67E7 | Alexa Fluor 647 | AB_10695877 | 647/Cy5 | link | |
| aSMA-660 | aSMA | *** | eBioscience | 50-9760-80 | 1A4 | eFluor 660 | AB_2574361 | 647/Cy5 | link | |
| B220-647 | CD45R/B220 | *** | BioLegend | 103226 | RA3-6B2 | Alexa Fluor 647 | AB_389330 | 647/Cy5 | link | |
| Bcl2-647 | Bcl2 | *** | BioLegend | 658705 | 100 | Alexa Fluor 647 | AB_2563279 | 647/Cy5 | link | |
| Catenin-647 | Beta-Catenin | *** | CST | 4627 | L54E2 | Alexa Fluor 647 | AB_10691326 | 647/Cy5 | link | |
| CD20-660 | CD20 | *** | eBioscience | 50-0202-80 | L26 | eFluor 660 | AB_11151691 | 647/Cy5 | link | |
| CD45-647 | CD45 | *** | BioLegend | 304020 | HI30 | Alexa Fluor 647 | AB_493034 | 647/Cy5 | link | |
| CD8a-660 | CD8 | *** | eBioscience | 50-0008-80 | AMC908 | eFluor 660 | AB_2574148 | 647/Cy5 | link | |
| CK5-647 | Cytokeratin 5 | *** | Abcam | AB193895 | EP1601Y | Alexa Fluor 647 | 647/Cy5 | link | ||
| CoIIV-647 | Collagen IV | *** | eBioscience | 51-9871-80 | 1042 | Alexa Fluor 647 | AB_10854267 | 647/Cy5 | link | |
| COXIV-647 | COXIV | *** | CST | 7561 | 3E11 | Alexa Fluor 647 | AB_10994876 | 647/Cy5 | link | |
| cPARP-647 | cPARP | *** | CST | 6987 | D64E10 | Alexa Fluor 647 | AB_10858215 | 647/Cy5 | link | |
| FOXA2-660 | FOXA2 | *** | eBioscience | 50-4778-82 | 3C10 | eFluor 660 | AB_2574221 | 647/Cy5 | link | |
| FOXP3-647 | FOXP3 | *** | BioLegend | 320113 | 206D | Alexa Fluor 647 | AB_439753 | 647/Cy5 | link | |
| gH2ax-647 | H2ax(S139) | *** | CST | 9720 | 20E3 | Alexa Fluor 647 | AB_10692910 | 647/Cy5 | link | |
| gH2ax-647 | H2ax(S139) | *** | BioLegend | 613407 | 2F3 | Alexa Fluor 647 | AB_2114994 | 647/Cy5 | link | |
| HES1-647 | HES1 | *** | Abcam | AB196577 | EPR4226 | Alexa Fluor 647 | 647/Cy5 | link | ||
| Ki67-647 | Ki67 | *** | CST | 12075 | D3B5 | Alexa Fluor 647 | 647/Cy5 | link | ||
| Ki67-647 | Ki67 | *** | BioLegend | 350509 | Ki-67 | Alexa Fluor 647 | AB_10900810 | 647/Cy5 | link | |
| mCD45-647 | ms_CD45 | *** | BioLegend | 103124 | 30-F11 | Alexa Fluor 647 | AB_493533 | 647/Cy5 | link | |
| mCD4-647 | ms_CD4 | *** | BioLegend | 100426 | GK1.5 | Alexa Fluor 647 | AB_493519 | 647/Cy5 | link | |
| mEPCAM-647 | ms_EPCAM | *** | BioLegend | 118211 | G8.8 | Alexa Fluor 647 | AB_1134104 | 647/Cy5 | link | |
| MHCI-647 | MHCI/HLAA | *** | Abcam | AB199837 | EP1395Y | Alexa Fluor 647 | 647/Cy5 | link | ||
| MHCII-647 | MHCII | *** | Abcam | AB201347 | EPR11226 | Alexa Fluor 647 | 647/Cy5 | link | ||
| mLy6C-647 | ms_Ly6C | *** | BioLegend | 128009 | HK1.4 | Alexa Fluor 647 | AB_1236551 | 647/Cy5 | link | |
| mTOR-647 | mTOR | *** | CST | 5048 | 7C10 | Alexa Fluor 647 | AB_10828101 | 647/Cy5 | link | |
| NFkB-647 | NFkB (p65) | *** | Abcam | AB190589 | E379 | Alexa Fluor 647 | 647/Cy5 | link | ||
| NGFR-647 | NGFR/CD271 | *** | Abcam | AB195180 | EP1039Y | Alexa Fluor 647 | 647/Cy5 | link | ||
| NUP98-647 | NUP98 | *** | CST | 13393 | C39A3 | Alexa Fluor 647 | 647/Cy5 | link | ||
| p21-647 | p21 | *** | CST | 8587 | 12D1 | Alexa Fluor 647 | AB_10892861 | 647/Cy5 | link | |
| p27-647 | p27 | *** | Abcam | AB194234 | Y236 | Alexa Fluor 647 | 647/Cy5 | link | ||
| pATM-660 | pATM(S1981) | *** | eBioscience | 50-9046-41 | 10H11.E12 | eFluor 660 | AB_2574312 | 647/Cy5 | link | |
| PAX8-647 | PAX8 | *** | Abcam | AB215953 | EPR18715 | Alexa Fluor 647 | 647/Cy5 | link | ||
| PDL1-647 | PD-L1/CD274 | *** | CST | 15005 | E1L3N | Alexa Fluor 647 | 647/Cy5 | link | ||
| pMK2-647 | pMK2(T334) | *** | CST | 4320 | 27B7 | Alexa Fluor 647 | AB_10695401 | 647/Cy5 | Discontinued | link |
| pmTOR-660 | pmTOR(S2448) | *** | eBioscience | 50-9718-41 | MRRBY | eFluor 660 | AB_2574351 | 647/Cy5 | link | |
| pS6_235-647 | pS6(S235/S236) | *** | CST | 4851 | D57.2.2E | Alexa Fluor 647 | AB_10695457 | 647/Cy5 | link | |
| pSTAT3-647 | pSTAT3(Y705) | *** | CST | 4324 | D3A7 | Alexa Fluor 647 | AB_10694637 | 647/Cy5 | link | |
| pTyr-647 | p-Tyrosine | *** | CST | 9415 | p-Tyr-100 | Alexa Fluor 647 | AB_10693160 | 647/Cy5 | link | |
| S100A4-647 | S100A4 | *** | Abcam | AB196168 | EPR2761(2) | Alexa Fluor 647 | 647/Cy5 | link | ||
| Survivin-647 | Survivin | *** | CST | 2866 | 71G4B7 | Alexa Fluor 647 | AB_10698609 | 647/Cy5 | link | |
| TUBB3-647 | TUBB3 | *** | BioLegend | 657405 | AA10 | Alexa Fluor 647 | AB_2563609 | 647/Cy5 | link | |
| Tubulin-647 | beta-Tubulin | *** | CST | 3624 | 9F3 | Alexa Fluor 647 | AB_10694204 | 647/Cy5 | link | |
| Vimentin-647 | Vimentin | *** | BioLegend | 677807 | O91D3 | Alexa Fluor 647 | AB_2616801 | 647/Cy5 | link | |
| anti-14-3-3 | 14-3-3 | *** | Santa Cruz | SC-629-G | Polyclonal | N/D | AB_630820 | N/D | link | |
| anti-53BP1 | 53BP1 | *** | Bethyl | A303-906A | Polyclonal | N/D | AB_2620256 | N/D | link | |
| anti-5HMC | 5HMC | *** | Active Motif | 39769 | Polyclonal | N/D | AB_10013602 | N/D | link | |
| anti-CD11b | CD11b | *** | Abcam | AB133357 | EPR1344 | N/D | AB_2650514 | N/D | link | |
| anti-CD2 | CD2 | *** | Abcam | AB37212 | Polyclonal | N/D | AB_726228 | N/D | link | |
| anti-CD20 | CD20 | *** | Dako | M0755 | L26 | N/D | AB_2282030 | N/D | link | |
| anti-CD3 | CD3 | *** | Dako | A0452 | Polyclonal | N/D | AB_2335677 | N/D | link | |
| anti-CD4 | CD4 | *** | Dako | M7310 | 4B12 | N/D | N/D | link | ||
| anti-CD45RO | CD45RO | *** | Dako | M0742 | UCHL1 | N/D | AB_2237910 | N/D | link | |
| anti-CD8 | CD8 | *** | Dako | M7103 | C8/144B | N/D | AB_2075537 | N/D | link | |
| anti-CycA2 | CycA2 | *** | Abcam | AB38 | E23.1 | N/D | AB_304084 | N/D | link | |
| anti-ET1 | ET-1 | *** | Abcam | AB2786 | TR.ET.48.5 | N/D | AB_303299 | N/D | link | |
| anti-FAP | FAP | *** | eBioscience | BMS168 | F11-24 | N/D | AB_10597443 | N/D | link | |
| anti-FOXP3 | FOXP3 | *** | BioLegend | 320102 | 206D | N/D | AB_430881 | N/D | link | |
| anti-LAMP2 | LAMP2 | *** | Abcam | AB25631 | H4B4 | N/D | AB_470709 | N/D | link | |
| anti-MCM6 | MCM6 | *** | Santa Cruz | SC-9843 | Polyclonal | N/D | AB_2142543 | N/D | link | |
| anti-PAX8 | PAX8 | *** | Abcam | AB191870 | EPR18715 | N/D | N/D | link | ||
| anti-PD1 | PD1 | *** | CST | 86163 | D4W2J | N/D | N/D | link | ||
| anti-pEGFR | pEGFR(Y1068) | *** | CST | 3777 | D7A5 | N/D | AB_2096270 | N/D | link | |
| anti-pERK | pERK(T202/Y204) | *** | CST | 4370 | D13.14.4E | N/D | AB_2315112 | N/D | link | |
| anti-pRB | pRB(S807/811) | *** | Santa Cruz | SC-16670 | Polyclonal | N/D | AB_655250 | N/D | Discontinued | link |
| anti-pRPA32 | pRPA32 (S4/S8) | *** | Bethyl | IHC-00422 | Polyclonal | N/D | AB_1659840 | N/D | link | |
| anti-pSTAT3 | pSTAT3 | *** | CST | 9145 | D3A7 | N/D | AB_2491009 | N/D | link | |
| anti-pTyr | pTyr | *** | CST | 9411 | p-Tyr-100 | N/D | AB_331228 | N/D | link | |
| anti-RPA32 | RPA32 | *** | Bethyl | IHC-00417 | Polyclonal | N/D | AB_1659838 | N/D | link | |
| anti-TPCN2 | TPCN2 | *** | NOVUSBIO | NBP1-86923 | Polyclonal | N/D | AB_11021735 | N/D | link | |
| anti-VEGFR1 | VEGFR1/FLT1 | *** | Santa Cruz | SC-31173 | Polyclonal | N/D | AB_2106885 | N/D | link | |
| Abeta-488 | Beta-Amyloid (1-16) | ** | BioLegend | 803013 | 6E10 | Alexa Fluor 488 | AB_2564765 | 488/FITC | link | |
| BRAF-FITC | B-RAF | ** | Abcam | ab175637 | K21-F | FITC | 488/FITC | link | ||
| BrdU-488 | BrdU | ** | BioLegend | 364105 | 3D4 | Alexa Fluor 488 | AB_2564499 | 488/FITC | link | |
| cCasp3-488 | cCasp3 | ** | R&D Systems | IC835G-025 | 269518 | Alexa Fluor 488 | 488/FITC | link | ||
| CD11b-488 | CD11b | ** | BioLegend | 101219 | M1/70 | Alexa Fluor 488 | AB_493545 | 488/FITC | link | |
| CD123-488 | CD123 | ** | BioLegend | 306035 | 6H6 | Alexa Fluor 488 | AB_2629569 | 488/FITC | link | |
| CD49b-FITC | CD49b | ** | BioLegend | 359305 | P1E6-C5 | FITC | AB_2562530 | 488/FITC | link | |
| CD69-FITC | CD69 | ** | BioLegend | 310904 | FN50 | FITC | AB_314839 | 488/FITC | link | |
| CD71-FITC | CD71 | ** | BioLegend | 334103 | CY1G4 | FITC | AB_1236432 | 488/FITC | link | |
| CD80-FITC | CD80 | ** | R&D Systems | FAB140F | 37711 | FITC | AB_357027 | 488/FITC | link | |
| CD8a-488 | CD8a | ** | eBioscience | 53-0086-41 | OKT8 | Alexa Fluor 488 | AB_10547060 | 488/FITC | link | |
| CDC2-FITC | CDC2/p34 | ** | Santa Cruz | SC-54 FITC | 17 | FITC | AB_627224 | 488/FITC | link | |
| CycB1-FITC | CycB1 | ** | Santa Cruz | SC-752 FITC | Polyclonal | FITC | AB_2072134 | 488/FITC | link | |
| FN-488 | Fibronection | ** | Abcam | AB198933 | F1 | Alexa Fluor 488 | 488/FITC | link | ||
| IFNG-488 | Interferron-Gamma | ** | BioLegend | 502517 | 4S.B3 | Alexa Fluor 488 | AB_493030 | 488/FITC | link | |
| IL1-FITC | IL1 | ** | BioLegend | 511705 | H1b-98 | FITC | AB_1236434 | 488/FITC | link | |
| IL6-FITC | IL6 | ** | BioLegend | 501103 | MQ2-13A5 | FITC | AB_315151 | 488/FITC | link | |
| mCD31-FITC | ms_CD31 | ** | eBioscience | 11-0311-82 | 390 | FITC | AB_465012 | 488/FITC | link | |
| mCD8a-488 | ms_CD8a | ** | BioLegend | 100726 | 53-6.7 | Alexa Fluor 488 | AB_493423 | 488/FITC | link | |
| Nestin-488 | Nestin | ** | eBioscience | 53-9843-80 | 10C2 | Alexa Fluor 488 | AB_1834347 | 488/FITC | link | |
| NeuN-488 | NeuN | ** | Millipore | MAB377X | A60 | Alexa Fluor 488 | AB_2149209 | 488/FITC | link | |
| PR-488 | PR/PGR | ** | Abcam | AB199224 | YR85 | Alexa Fluor 488 | 488/FITC | link | ||
| Snail1-488 | Snail1 | ** | eBioscience | 53-9859-80 | 20C8 | Alexa Fluor 488 | AB_2574482 | 488/FITC | link | |
| TGFB-FITC | TGFB1 | ** | BioLegend | 349605 | TW4-2F8 | FITC | AB_10679043 | 488/FITC | link | |
| TNFa-488 | TNFa | ** | BioLegend | 502917 | MAb11 | Alexa Fluor 488 | AB_493122 | 488/FITC | link | |
| AR-555 | AR | ** | CST | 8956 | D6F11 | Alexa Fluor 555 | AB_11129223 | 555/Cy3 | link | |
| CD11a-PE | CD11a | ** | BioLegend | 301207 | HI111 | PE | AB_314145 | 555/Cy3 | link | |
| CD11b-555 | CD11b | ** | Abcam | AB206616 | EPR1344 | Alexa Fluor 555 | 555/Cy3 | link | ||
| CD131-PE | CD131 | ** | BD | 559920 | JORO50 | PE | AB_397374 | 555/Cy3 | link | |
| CD14-PE | CD14 | ** | eBioscience | 12-0149 | 61D3 | PE | AB_10597598 | 555/Cy3 | link | |
| CD1a-PE | CD1a | ** | BioLegend | 300105 | HI149 | PE | AB_314019 | 555/Cy3 | link | |
| CD1c-PE | CD1c | ** | BioLegend | 331505 | L161 | PE | AB_1089000 | 555/Cy3 | link | |
| CD20-PE | CD20 | ** | BioLegend | 302305 | 2H7 | PE | AB_314253 | 555/Cy3 | link | |
| CD23-PE | CD23 | ** | eBioscience | 12-0232-81 | B3B4 | PE | AB_465592 | 555/Cy3 | link | |
| CD31-PE | CD31 | ** | eBioscience | 12-0319-41 | WM-59 | PE | AB_10670623 | 555/Cy3 | link | |
| CD31-PE | CD31 | ** | R&D Systems | FAB3567P-025 | 9G11 | PE | AB_2279388 | 555/Cy3 | link | |
| CD34-PE | CD34 | ** | Abcam | AB30377 | QBEND/10 | PE | AB_726407 | 555/Cy3 | link | |
| CD45R-e570 | CD45R/B220 | ** | eBioscience | 41-0452-80 | RA3-6B2 | eFluor 570 | AB_2573598 | 555/Cy3 | link | |
| CD71-PE | CD71 | ** | eBioscience | 12-0711-81 | R17217 | PE | AB_465739 | 555/Cy3 | link | |
| CD86-PE | CD86 | ** | BioLegend | 305405 | IT2.2 | PE | AB_314525 | 555/Cy3 | link | |
| CK19-570 | Cytokeratin 19 | ** | eBioscience | 41-9898-80 | BA17 | eFluor 570 | AB_11218678 | 555/Cy3 | link | |
| HER2-570 | HER2 | ** | eBioscience | 41-9757-80 | MJD2 | eFluor 570 | AB_2573628 | 555/Cy3 | link | |
| IL3-PE | IL3 | ** | BD | 554383 | MP2-8F8 | PE | AB_395358 | 555/Cy3 | link | |
| NFATc1-PE | NFATc1 | ** | BioLegend | 649605 | 7A6 | PE | AB_2562546 | 555/Cy3 | link | |
| PDL1-PE | PD-L1/CD274 | ** | BioLegend | 329705 | 29E.2A3 | PE | AB_940366 | 555/Cy3 | link | |
| pMAPK (T202/Y204) | pERK1/2(T202/Y204) | ** | CST | 14095 | 197G2 | PE | 555/Cy3 | link | ||
| pMAPK (Y204/Y187) | pERK1/2(Y204/Y187) | ** | CST | 75165 | D1H6G | PE | 555/Cy3 | link | ||
| pSTAT1-PE | pSTAT1(Y705) | ** | BioLegend | 686403 | A15158B | PE | AB_2616938 | 555/Cy3 | link | |
| ABCC1-647 | ABCC1 | ** | BioLegend | 370203 | QCRL-2 | Alexa Fluor 647 | AB_2566664 | 647/Cy5 | link | |
| AnnexinV-674 | N/D | ** | BioLegend | 640911 | NA | Alexa Fluor 647 | AB_2561293 | 647/Cy5 | link | |
| CD103-647 | CD103 | ** | BioLegend | 350209 | Ber-ACT8 | Alexa Fluor 647 | AB_10640870 | 647/Cy5 | link | |
| CD25-647 | CD25 | ** | BioLegend | 302617 | BC96 | Alexa Fluor 647 | AB_493046 | 647/Cy5 | link | |
| CD31-APC | CD31 | ** | eBioscience | 17-0319-41 | WM-59 | APC | AB_10853188 | 647/Cy5 | link | |
| CD68-APC | CD68 | ** | BioLegend | 333809 | Y1/82A | APC | AB_10567107 | 647/Cy5 | link | |
| CD8a-647 | CD8a | ** | BioLegend | 344725 | SK1 | Alexa Fluor 647 | AB_2563451 | 647/Cy5 | link | |
| CD8a-647 | CD8a | ** | R&D Systems | FAB1509R-025 | 37006 | Alexa Fluor 647 | 647/Cy5 | link | ||
| CycE-660 | CycE | ** | eBioscience | 50-9714-80 | HE12 | eFluor 660 | AB_2574350 | 647/Cy5 | link | |
| HIF1-647 | HIF1 | ** | BioLegend | 359705 | 546-16 | Alexa Fluor 647 | AB_2563331 | 647/Cy5 | link | |
| HP1-647 | HP1 | ** | Abcam | AB198391 | EPR5777 | Alexa Fluor 647 | 647/Cy5 | link | ||
| mCD123-APC | ms_CD123 | ** | eBioscience | 17-1231-81 | 5B11 | APC | AB_891363 | 647/Cy5 | link | |
| NGFR-647 | NGFR/CD271 | ** | BD | 560326 | C40-1457 | Alexa Fluor 647 | AB_1645403 | 647/Cy5 | link | |
| pBTK-660 | pBTK(Y551/Y511) | ** | eBioscience | 50-9015-80 | M4G3LN | eFluor 660 | AB_2574306 | 647/Cy5 | link | |
| PD1-647 | PD1 | ** | Abcam | AB201825 | EPR4877 (2) | Alexa Fluor 647 | 647/Cy5 | link | ||
| PR-660 | PR/PGR | ** | eBioscience | 50-9764-80 | KMC912 | eFluor 660 | AB_2574363 | 647/Cy5 | link | |
| RUNX3-660 | RUNX3 | ** | eBioscience | 50-9817-80 | R3-5G4 | eFluor 660 | AB_2574383 | 647/Cy5 | link | |
| SOX2-647 | SOX2 | ** | Abcam | AB192075 | Polyclonal | Alexa Fluor 647 | 647/Cy5 | link | ||
| anti-53BP1 | 53BP1 | ** | Millipore | MAB3802 | BP13 | N/D | AB_2206767 | N/D | link | |
| anti-Axl | Axl | ** | R&D | AF154 | Polyclonal | N/D | AB_354852 | N/D | link | |
| anti-CD11b | CD11b | ** | Abcam | AB52478 | EP1345Y | N/D | AB_868788 | N/D | link | |
| anti-CD8a | CD8 | ** | eBioscience | 14-0085-80 | C8/144B | N/D | AB_11151339 | N/D | link | |
| anti-CEP170 | CEP170 | ** | Abcam | AB72505 | Polyclonal | N/D | AB_1268101 | N/D | link | |
| anti-cMYC | cMYC | ** | BioLegend | 626801 | 9E10 | N/D | AB_2235686 | N/D | link | |
| anti-CPS1 | CPS1 | ** | Abcam | AB129076 | EPR7493-3 | N/D | AB_11156290 | N/D | link | |
| anti-E2F1 | E2F1 | ** | ThermoFisher | MS-879-P1 | KH95 | N/D | AB_143934 | N/D | link | |
| anti-eEF2K | eEF2K | ** | Santa Cruz | SC-21642 | K-19 | N/D | AB_640043 | N/D | link | |
| anti-Emil1 | Emil1 | ** | Abcam | AB212397 | EMIL/1176 | N/D | N/D | link | ||
| anti-FKHRL1 | FKHRL1 | ** | Santa Cruz | SC-9812 | Polyclonal | N/D | AB_640608 | N/D | Discontinued | link |
| anti-FLAG | FLAG | ** | Sigma | F1804 | M2 | N/D | AB_262044 | N/D | link | |
| anti-GranB | Granzyme_B | ** | Dako | M7235 | M7235 | N/D | AB_2114697 | N/D | link | |
| anti-HMB45 | HMB45 | ** | Abcam | AB732 | HMB45+M2-7C10+M2-9E3 | N/D | AB_305844 | N/D | link | |
| anti-HSP90b | HSP90b | ** | Santa Cruz | SC-1057 | D-19 | N/D | AB_2121392 | N/D | link | |
| anti-IL2Ra | IL2Ra | ** | Abcam | AB128955 | EPR6452 | N/D | AB_11141054 | N/D | link | |
| anti-LAMP2 | LAMP2 | ** | R&D | AF6228 | Polyclonal | N/D | AB_10971818 | N/D | link | |
| anti-MITF | MITF | ** | Abcam | AB12039 | C5 | N/D | AB_298801 | N/D | link | |
| anti-Ncad | N-Cadherin | ** | Abcam | AB18203 | Polyclonal | N/D | AB_444317 | N/D | link | |
| anti-NCAM | NCAM | ** | Abcam | AB6123 | ERIC-1 | N/D | AB_2149537 | N/D | link | |
| anti-NF1 | NF1 | ** | Abcam | AB178323 | McNFn27b | N/D | N/D | link | ||
| anti-pCTD | Pol II CTD(S2) | ** | Active Motif | 61083 | 3E10 | N/D | AB_2687450 | N/D | link | |
| anti-PD1 | PD1 | ** | CST | 43248 | EH33 | N/D | N/D | link | ||
| anti-pTuberin | pTuberin(S664) | ** | Abcam | AB133465 | EPR8202 | N/D | AB_11157389 | N/D | link | |
| anti-S100 | S100 | ** | Dako | Z0311 | Polyclonal | N/D | AB_10013383 | N/D | link | |
| anti-SIRT3 | SIRT3 | ** | CST | 2627 | C73E3 | N/D | AB_2188622 | N/D | link | |
| anti-TIA1 | TIA1 | ** | Santa Cruz | SC-1751 | Polyclonal | N/D | AB_2201433 | N/D | link | |
| anti-TLR3 | TLR3 | ** | Santa Cruz | SC-8691 | Polyclonal | N/D | AB_2240700 | N/D | link | |
| anti-TNFa | TNFa | ** | Abcam | AB11564 | MP6-XT3 | N/D | AB_298170 | N/D | link | |
| anti-TPCN2 | TPCN2 | ** | Abcam | AB119915 | Polyclonal | N/D | AB_10903692 | N/D | link | |
| CD11a-FITC | CD11a | * | eBioscience | 11-0119-41 | HI111 | FITC | AB_10597888 | 488/FITC | link | |
| CD20-FITC | CD20 | * | BioLegend | 302303 | 2H7 | FITC | AB_314251 | 488/FITC | link | |
| CD2-FITC | CD2 | * | BioLegend | 300206 | RPA-2.10 | FITC | AB_314030 | 488/FITC | link | |
| CD45RO-488 | CD45RO | * | BioLegend | 304212 | UCHL1 | Alexa Fluor 488 | AB_528823 | 488/FITC | link | |
| CD8a-488 | CD8 | * | BioLegend | 301024 | RPA-T8 | Alexa Fluor 488 | AB_2561282 | 488/FITC | link | |
| cJUN-FITC | cJUN | * | Santa Cruz | SC-1694 FITC | Polyclonal | FITC | AB_631263 | 488/FITC | Discontinued | link |
| CXCR5-FITC | CXCR5 | * | BioLegend | 356913 | J252D4 | FITC | AB_2561895 | 488/FITC | link | |
| Ecad-FITC | Ecad | * | BioLegend | 324103 | 67A4 | FITC | AB_756065 | 488/FITC | link | |
| FOXP3-488 | FOXP3 | * | BioLegend | 320011 | 150D | Alexa Fluor 488 | AB_439747 | 488/FITC | link | |
| MITF-488 | MITF | * | Novus Biologicals | NB100-56561AF488 | 21D1418 | Alexa Fluor 488 | AB_838580 | 488/FITC | link | |
| NCAM-488 | NCAM/CD56 | * | Abcam | AB200333 | EPR2566 | Alexa Fluor 488 | 488/FITC | link | ||
| NCAM-FITC | NCAM/CD56 | * | ThermoFisher | 11-0566-41 | TULY56 | FITC | AB_2572458 | 488/FITC | link | |
| NGFR-FITC | NGFR/CD271 | * | BioLegend | 345103 | ME20.4 | FITC | AB_1937226 | 488/FITC | link | |
| PD1-488 | PD-1 | * | BioLegend | 367407 | NAT105 | Alexa Fluor 488 | AB_2566677 | 488/FITC | link | |
| PD1-488 | PD-1 | * | BioLegend | 329935 | EH12.2H7 | Alexa Fluor 488 | AB_2563593 | 488/FITC | link | |
| pERK-488 | pERK(T202/Y204) | * | CST | 4374 | E10 | Alexa Fluor 488 | AB_10705598 | 488/FITC | link | |
| pERK-488 | pERK(T202/Y204) | * | CST | 4780 | 137F5 | Alexa Fluor 488 | AB_10705598 | 488/FITC | link | |
| S100A4-FITC | S100A4 | * | BioLegend | 370007 | NJ-4F3-D1 | FITC | AB_2572073 | 488/FITC | link | |
| SOX2-488 | SOX2 | * | BioLegend | 656109 | 14A6A34 | Alexa Fluor 488 | AB_2563956 | 488/FITC | link | |
| CD133-PE | CD133 | * | eBioscience | 12-1338-41 | TMP4 | PE | AB_1582258 | 555/Cy3 | link | |
| cMyc-TRITC | cMYC | * | Santa Cruz | SC-40 TRITC | 9E10 | TRITC | AB_627268 | 555/Cy3 | link | |
| cPARP-555 | cPARP | * | CST | 6894 | D64E10 | Alexa Fluor 555 | AB_10830735 | 555/Cy3 | link | |
| CTLA4-PE | CTLA4 | * | BioLegend | 369603 | BNI3 | PE | AB_2566796 | 555/Cy3 | link | |
| GATA3-594 | GATA3 | * | BioLegend | 653816 | 16E10A23 | Alexa Fluor 594 | AB_2563353 | 555/Cy3 | link | |
| GFAP-Cy3 | GFAP | * | Millipore | MAB3402C3 | NA | Cy3 | AB_11213580 | 555/Cy3 | link | |
| Oct4-555 | OCT_4 | * | CST | 4439 | C30A3 | Alexa Fluor 555 | AB_10922586 | 555/Cy3 | link | |
| p21-555 | p21 | * | CST | 8493 | 12D1 | Alexa Fluor 555 | AB_10860074 | 555/Cy3 | link | |
| PD1-PE | PD1 | * | BioLegend | 329905 | EH12.2H7 | PE | AB_940481 | 555/Cy3 | link | |
| PDGFRb-555 | PDGFRb | * | Abcam | AB206874 | Y92 | Alexa Fluor 555 | 555/Cy3 | link | ||
| pSTAT1-555 | pSTAT1 | * | CST | 8183 | 58D6 | Alexa Fluor 555 | AB_10860600 | 555/Cy3 | link | |
| TIM1-PE | TIM1 | * | BioLegend | 353903 | 1D12 | PE | AB_11125165 | 555/Cy3 | link | |
| cCasp3-647 | cCasp3 | * | CST | 9602 | D3E9 | Alexa Fluor 647 | AB_2687881 | 647/Cy5 | link | |
| CD103-APC | CD103 | * | eBioscience | 17-1038-41 | B-Ly7 | APC | AB_10669816 | 647/Cy5 | link | |
| CD3-647 | CD3 | * | BioLegend | 300422 | UCHT1 | Alexa Fluor 647 | AB_493092 | 647/Cy5 | link | |
| CD3-660 | CD3 | * | eBioscience | 50-0037-41 | OKT3 | eFluor 660 | AB_2574150 | 647/Cy5 | link | |
| CD3-APC | CD3 | * | eBioscience | 17-0038-41 | UCHT1 | APC | AB_10804761 | 647/Cy5 | link | |
| CD45RO-APC | CD45RO | * | BioLegend | 304210 | UCHL1 | APC | AB_314426 | 647/Cy5 | link | |
| ER-647 | ER | * | Abcam | AB205851 | EPR4097 | Alexa Fluor 647 | 647/Cy5 | link | ||
| FOXO3a-647 | FOXO3a | * | Abcam | AB196539 | EP1949Y | Alexa Fluor 647 | 647/Cy5 | link | ||
| GZMA-e660 | granzyme A | * | ThermoFisher | 50-9177-41 | CB9 | eFluor 660 | AB_2574330 | 647/Cy5 | link | |
| GZMB-647 | Granzyme_B | * | BioLegend | 515405 | GB11 | Alexa Fluor 647 | AB_2294995 | 647/Cy5 | link | |
| GZMB-APC | Granzyme_B | * | R&D Systems | IC29051A | 356412 | APC | AB_894691 | 647/Cy5 | Discontinued | link |
| HER2-647 | HER2 | * | BioLegend | 324412 | 24D2 | Alexa Fluor 647 | AB_2262300 | 647/Cy5 | link | |
| mCD49b-647 | ms_CD49b | * | BioLegend | 103511 | HMα2 | Alexa Fluor 647 | AB_528830 | 647/Cy5 | link | |
| NCAM-647 | NCAM/CD56 | * | BioLegend | 362513 | 5.1H11 | Alexa Fluor 647 | AB_2564086 | 647/Cy5 | link | |
| NCAM-e660 | NCAM/CD56 | * | ThermoFisher | 50-0565-80 | 5tukon56 | eFluor 660 | AB_2574160 | 647/Cy5 | link | |
| pAKT-647 | pAKT | * | CST | 4075 | D9E | Alexa Fluor 647 | AB_10691856 | 647/Cy5 | link | |
| pERK-647 | pERK (T202/Y204) | * | CST | 4375 | E10 | Alexa Fluor 647 | AB_10706777 | 647/Cy5 | link | |
| pERK-647 | pERK (T202/Y204) | * | BioLegend | 369503 | 6B8B69 | Alexa Fluor 647 | AB_2571895 | 647/Cy5 | link | |
| pIKBa-660 | pIKBa | * | eBioscience | 50-9035-41 | RILYB3R | eFluor 660 | AB_2574310 | 647/Cy5 | link | |
| YAP-647 | YAP | * | CST | 38707S | D8H1X | Alexa Fluor 647 | 647/Cy5 | link | ||
| anit-FANCD2 | FANCD2 | * | Bethyl | IHC-00624 | Polyclonal | N/D | AB_10752755 | N/D | link | |
| anit-pcJUN | p-cJUN | * | Santa Cruz | SC-822 | KM-1 | N/D | AB_627262 | N/D | link | |
| anti-AXL | AXL | * | CST | 8661 | C89E7 | N/D | AB_11217435 | N/D | link | |
| anti-CXCR5 | CXCR5 | * | GeneTex | GTX100351 | Polyclonal | N/D | AB_1240668 | N/D | link | |
| anti-CXCR5 | CXCR5 | * | R&D | MAB-190-SP | 51505 | N/D | AB_2292654 | N/D | link | |
| anti-FOXO3a | FOXO3a | * | CST | 2497 | 75D8 | N/D | AB_836876 | N/D | link | |
| anti-GZMB | Granzyme B | * | Abcam | AB4059 | Polyclonal | N/D | AB_304251 | N/D | link | |
| anti-PD1 | PD-1 | * | Abcam | AB63477 | Polyclonal | N/D | AB_2159165 | N/D | link | |
| anti-PD1 | PD-1 | * | ThermoFisher | 14-9985-81 | J43 | N/D | AB_468663 | N/D | link | |
| anti-PD1 | PD-1 | * | R&D | AF1021 | Polyclonal | N/D | AB_354541 | N/D | link | |
| anti-RFP | RFP | * | ThermoFisher | R10367 | Polyclonal | N/D | AB_2315269 | N/D | link | |
| CD11C-BV570 | CD11C | * | BioLegend | 117331 | N418 | BV570 | AB_10900261 | N/D | link | |
| CD45-BV785 | CD45 | * | BioLegend | 304047 | HI30 | BV785 | AB_2563128 | N/D | link | |
| LY6G-BV570 | LY6G | * | BioLegend | 127629 | 1A8 | BV570 | AB_10899738 | N/D | link |
*** Show positive/correct signals in mutliple samples/tissues as judged by eye
** Show positive/correct signals in some but not all samples tested as judged by eye
* show no signal or obviously incorrect signals in all samples tested
