Not Currently Recommended Antibodies
Last updated: February 2021
We have tested a number of antibodies in human FFPE tissue specimens against different proteins for their compatibility with t-CyCIF. This list includes antibodies that are recommended for t-CyCIF; they demonstrate expected staining pattern in multiple tissue specimens.
- 1 star: Demonstrate expected and unexpected staining pattern in multiple tissue specimens.
- 0 stars: Demonstrate unexpected staining pattern or no signal in multiple tissue specimens tested.
Note: Performance does not infer validation.
You can browse, search, and sort (by clicking the table column heading) the following table.
Search Antibodies
| Target Protein | Fluorophore | Performance (Human) | Vendor | Catalog Number | Clone | RRID | Note |
|---|---|---|---|---|---|---|---|
| 53BP1 | * | Cell Signaling Technology | 4937 | AB_10694558 | |||
| ACTH | Alexa Fluor 647 | * | Novus Biologicals | NBP2-34529AF647 | 2F6 | AB_2889346 | |
| AKT (pS473) | Alexa Fluor 647 | * | Cell Signaling Technology | 2337 | 193H12 | AB_490782 | |
| Alpha-actin-2 | Alexa Fluor 750 | * | R&D Systems | IC1420S-025 | 1A4 | AB_2868436 | |
| Annexin 5 | Alexa Fluor 647 | * | BioLegend | 640911 | |||
| AREB6 | Alexa Fluor 647 | * | Abcam | ab216121 | EPR17375 | AB_2868420 | |
| AXL | phycoerythrin | * | Cell Signaling Technology | 78909 | C89E7 | AB_2799921 | |
| BANF1 | Alexa Fluor 647 | * | Abcam | ab208621 | EPR7668 | AB_2868493 | |
| BANF1 | Alexa Fluor 568 | * | Abcam | ab208534 | EPR7668 | AB_2868492 | |
| BCLXL | Alexa Fluor 647 | * | Cell Signaling Technology | 86387 | 54H6 | AB_2800078 | |
| BIM | phycoerythrin | * | Cell Signaling Technology | 12186 | C34C5 | AB_2797842 | |
| BrdU | Alexa Fluor 488 | * | BioLegend | 364105 | 3D4 | AB_2564499 | |
| BTK (pY551; pY511) | eFluor 660 | * | Thermo Fisher Scientific | 50-9015-80 | M4G3LN | AB_2574306 | Discontinued |
| c-Jun | Alexa Fluor 488 | * | Abcam | ab193780 | E254 | AB_2728783 | Discontinued |
| c-Myc | * | BioLegend | 626801 | 9E10 | AB_2235686 | ||
| Calponin | Alexa Fluor 555 | * | Abcam | ab208051 | EP798Y | Discontinued | |
| Catenin beta-1 | Alexa Fluor 488 | * | BD Biosciences | 562505 | 14 | AB_11154224 | |
| CCNA | phycoerythrin | * | Santa Cruz Biotechnology | sc-271682 PE | AB_10709300 | ||
| CD10 | FITC | * | Thermo Fisher Scientific | MA5-16943 | SN5c | AB_2538419 | |
| CD10 | Alexa Fluor 488 | * | Abcam | ab225479 | EPR5904-110 | AB_2884941 | |
| CD10 | * | Abcam | ab951 | 56C6 | AB_2146533 | ||
| CD103 | Alexa Fluor 647 | * | BioLegend | 350209 | Ber-ACT8 | AB_10640870 | |
| CD103 | * | Cell Marque (Millipore Sigma) | 437R-15 | EP206 | AB_2884943 | ||
| CD103 | * | Abcam | ab129202 | EPR4166(2) | AB_11142856 | ||
| CD115 | * | Abcam | ab183316 | SP211 | AB_2885197 | ||
| CD11a | phycoerythrin | * | BioLegend | 301207 | HI111 | AB_314145 | |
| CD11b | Alexa Fluor 555 | * | Abcam | ab206616 | EPR1344 | AB_2728809 | |
| CD11b | Alexa Fluor 488 | * | BioLegend | 101219 | M1/70 | AB_493545 | |
| CD11c | * | Thermo Fisher Scientific | 14-9761-82 | 118/A5 | AB_2572997 | ||
| CD123 | APC | * | Thermo Fisher Scientific (eBioscience) | 17-1231-81 | 5B11 | AB_891363 | Discontinued |
| CD123 | Alexa Fluor 488 | * | BioLegend | 306035 | 6H6 | AB_2629569 | |
| CD131 | phycoerythrin | * | BD Biosciences | 559920 | JORO50 | AB_397374 | |
| CD134 | FITC | * | Thermo Fisher Scientific | 11-1347-41 | ACT-35 | AB_10596491 | |
| CD14 | phycoerythrin | * | Thermo Fisher Scientific | 12-0149-41 | 61D3 | AB_10597598 | |
| CD163 | * | Abcam | ab87099 | AB_11154711 | |||
| CD166 | Alexa Fluor 488 | * | Abcam | ab197543 | EPR2759(2) | AB_2889210 | |
| CD1a | phycoerythrin | * | BioLegend | 300105 | HI149 | AB_314019 | |
| CD1c | phycoerythrin | * | BioLegend | 331505 | L161 | AB_1089000 | |
| CD2 | * | Abcam | ab37212 | AB_726228 | |||
| CD20 | phycoerythrin | * | BioLegend | 302305 | 2H7 | AB_314253 | |
| CD209 | * | Santa Cruz Biotechnology | sc-65740 | DC28 | AB_1121347 | ||
| CD23 | phycoerythrin | * | Thermo Fisher Scientific | 12-0232 | B3B4 | AB_465595 | |
| CD24 | FITC | * | Abcam | AB_726282 | SN3 | AB_726282 | |
| CD25 | Alexa Fluor 647 | * | BioLegend | 302617 | BC96 | AB_493046 | |
| CD25 | * | Abcam | ab128955 | EPR6452 | AB_11141054 | ||
| CD273 | * | Cell Signaling Technology | 82723 | D7U8C | AB_2799999 | ||
| CD278 | * | Cell Signaling Technology | 89601 | D1K2T | AB_2800142 | ||
| CD278 | * | Abcam | ab105227 | SP98 | AB_10710236 | ||
| CD303 | * | Millipore Sigma | MABF94 | 10E6.1 | AB_2889343 | ||
| CD305 | Alexa Fluor 647 | * | BioLegend | 342802 | NKTA255 | AB_2133480 | |
| CD31 | phycoerythrin | * | Thermo Fisher Scientific | 12-0319-41 | WM-59 | AB_10670623 | |
| CD31 | phycoerythrin | * | Abcam | ab215660 | JC/70A | AB_2889211 | |
| CD31 | FITC | * | Thermo Fisher Scientific (eBioscience) | 11-0311-82 | 390 | AB_465012 | |
| CD31 | APC | * | Thermo Fisher Scientific | 17-0319-41 | WM-59 | AB_10853188 | |
| CD34 | phycoerythrin | * | Abcam | ab30377 | QBEND/10 | AB_726407 | |
| CD39 | * | Abcam | ab223842 | EPR20627 | AB_2889212 | ||
| CD40 | * | Cell Signaling Technology | 40868 | D8W3N | AB_2799188 | ||
| CD45 | * | BioLegend | 304047 | HI30 | AB_2563128 | ||
| CD45RA | Alexa Fluor 488 | * | BioLegend | 304114 | HI100 | AB_528816 | |
| CD45RO | APC | * | BioLegend | 304210 | UCHL1 | AB_314426 | |
| CD49a | * | R&D Systems | AF5676 | AB_2129238 | |||
| CD49b | FITC | * | BioLegend | 359305 | P1E6-C5 | AB_2562530 | |
| CD49b | Alexa Fluor 647 | * | BioLegend | 103511 | HMa2 | AB_528830 | |
| CD49b | Alexa Fluor 647 | * | BioLegend | 108912 | DX5 | AB_492880 | |
| CD56 | * | Abcam | ab6123 | ERIC-1 | AB_2149537 | ||
| CD68 | APC | * | BioLegend | 333809 | Y1/82A | AB_10567107 | |
| CD69 | FITC | * | BioLegend | 310904 | FN50 | AB_314839 | |
| CD71 | phycoerythrin | * | Thermo Fisher Scientific | 12-0711 | R17217 | AB_465739 | |
| CD71 | FITC | * | BioLegend | 334103 | CY1G4 | AB_1236432 | |
| CD73 | * | Abcam | ab124725 | EPR6115 | AB_10976033 | Discontinued | |
| CD8 | Alexa Fluor 647 | * | BioLegend | 344725 | SK1 | AB_2563451 | |
| CD8 | Alexa Fluor 647 | * | R&D Systems | FAB1509R-025 | 37006 | AB_2728841 | |
| CD80 | FITC | * | R&D Systems | FAB140F | 37711 | AB_357027 | |
| CD86 | phycoerythrin | * | BioLegend | 305405 | IT2.2 | AB_314525 | |
| CD8a | Alexa Fluor 488 | * | Thermo Fisher Scientific (eBioscience) | 53-0086-41 | OKT8 | AB_10547060 | |
| CD8a | Alexa Fluor 488 | * | BioLegend | 103511 | 53-6.7 | AB_493423 | |
| CD8a | * | Thermo Fisher Scientific | 14-0085 | C8/144B | AB_11151339 | ||
| CDC2 | FITC | * | Santa Cruz Biotechnology | sc-54 FITC | 17 | AB_2074908 | |
| CDK1/2/3 (pT14) | Alexa Fluor 488 | * | Abcam | ab193829 | E161 | ||
| CDK4 | phycoerythrin | * | Cell Signaling Technology | 42749 | D9G3E | AB_2799229 | |
| CDK4 | * | Abcam | ab108357 | EPR4513-32-7 | AB_10867218 | ||
| CDX2 | Alexa Fluor 647 | * | Abcam | ab195008 | EPR2764Y | AB_2889213 | |
| Checkpoint kinase 1 (pS296) | * | MBL International | D313-3 | 44-10-8 | AB_11161214 | ||
| Cleaved Caspase 3 (D175) | Alexa Fluor 488 | * | Cell Signaling Technology | 9669S | Asp175 | AB_10694067 | |
| Cleaved Caspase 3 (D175) | Alexa Fluor 647 | * | Cell Signaling Technology | 9602 | D3E9 | AB_2687881 | |
| Cleaved Caspase 3 (D214) | Alexa Fluor 488 | * | Cell Signaling Technology | 9603 | D3E9 | AB_11179205 | |
| Cleaved PARP (D214) | Alexa Fluor 647 | * | Cell Signaling Technology | 6987 | D64E10 | AB_10858215 | |
| COX IV-1 | Alexa Fluor 488 | * | Cell Signaling Technology | 4853 | 3E11 | AB_2261052 | |
| CPS1 | * | Abcam | ab129076 | EPR7493-3 | AB_11156290 | ||
| CPT1A | Alexa Fluor 488 | * | Abcam | ab171449 | 8F6AE9 | AB_2714024 | |
| Cyclin-E1 | eFluor 660 | * | Thermo Fisher Scientific (eBioscience) | 50-9714-80 | HE12 | AB_2574350 | |
| CytC | Alexa Fluor 647 | * | Abcam | ab192486 | EPR1327 | AB_2889214 | |
| Cytokeratin 14 | Alexa Fluor 647 | * | Abcam | ab206100 | EPR17350 | AB_2889215 | |
| Cytokeratin 19 | eFluor 570 | * | Thermo Fisher Scientific | 41-9898-80 | BA17 | AB_11218678 | |
| Cytokeratin 5 | Alexa Fluor 488 | * | Abcam | ab207351 | EPR1600Y | AB_2889216 | |
| Cytokeratin 8 | Alexa Fluor 488 | * | Abcam | ab192467 | EP1628Y | AB_2864346 | |
| E-cadherin | Alexa Fluor 647 | * | Abcam | ab194982 | EP700Y | AB_2889217 | |
| E2F-1 | Alexa Fluor 555 | * | Abcam | AB208078 | EPR3818(3) | Discontinued | |
| E2F-1 | * | Thermo Fisher Scientific | MS-879-P1 | KH95 | AB_143934 | Discontinued | |
| eEF2K | * | Santa Cruz Biotechnology | sc-21642 | K-19 | AB_640043 | ||
| Emil1 | * | Abcam | ab18341 | AB_444432 | |||
| EpCAM | FITC | * | Abcam | ab8666 | B29.1 (VU-1D9) | AB_306701 | |
| ERK | * | Cell Signaling Technology | 4696 | L34F12 | AB_390780 | ||
| ERK (pT202; pY204) | Alexa Fluor 647 | * | Cell Signaling Technology | 4375 | E10 | AB_10706777 | |
| ERK-1 (pT202; pY204) | * | Cell Signaling Technology | 4370 | D13.14.4E | AB_2297462 | ||
| ET1 | * | Abcam | ab2786 | TR.ET.48.5 | AB_303299 | ||
| EZHZ2 | Alexa Fluor 647 | * | Cell Signaling Technology | 45638 | D2C9 | AB_2799287 | |
| FANCD2 | Alexa Fluor 647 | * | Abcam | ab200763 | EPR2302 | AB_2889218 | |
| FANCD2 | * | Bethyl Laboratories Inc. | IHC-00624 | AB_10752755 | |||
| FLAG | * | Millipore Sigma | F1804 | M2 | AB_262044 | ||
| FN | Alexa Fluor 488 | * | Abcam | ab198933 | F1 | AB_2728807 | |
| FOXJ1 | * | Thermo Fisher Scientific | PA5-36210 | AB_2553418 | |||
| FOXM1 | * | Cell Signaling Technology | 5436S | D12D5 | AB_10692483 | ||
| FOXM1 | * | Millipore Sigma | HPA029974 | AB_10670030 | |||
| FOXO3 | * | Santa Cruz Biotechnology | sc-9812 | S-20 | AB_640608 | ||
| Gamma Tubulin | Alexa Fluor 647 | * | Abcam | ab191114 | TU-30 | AB_2889219 | |
| Gamma Tubulin | Alexa Fluor 488 | * | Abcam | ab205475 | EPR16793 | AB_2889220 | |
| GATA4 | Alexa Fluor 647 | * | Abcam | ab194072 | EPR4768 | Discontinued | |
| GLUT1 | Alexa Fluor 647 | * | Abcam | ab195020 | EPR3915 | AB_2783877 | |
| Granzyme A | APC | * | R&D Systems | IC29051A | 356412 | AB_894691 | |
| Granzyme B | * | Abcam | ab134933 | EPR8260 | AB_2889221 | ||
| Granzyme B | * | Abcam | ab4059 | AB_304251 | |||
| HER3 | phycoerythrin | * | Cell Signaling Technology | 77956 | D22C5 | AB_2799907 | |
| HGH | Alexa Fluor 647 | * | Novus Biologicals | NB500-364 AF647 | GH-45 | AB_10001069 | |
| Histone H2A.X (pS139) | phycoerythrin | * | BioLegend | 613412 | 2F3 | AB_2616871 | |
| Histone H2A.X (pS139) | Alexa Fluor 555 | * | Cell Signaling Technology | 8228S | 20E3 | AB_10858874 | Discontinued |
| Histone H2B | * | Santa Cruz Biotechnology | sc-8650 | AB_2118153 | Discontinued | ||
| Histone H3.1 (K27me3) | Alexa Fluor 647 | * | Cell Signaling Technology | 12158 | C36B11 | AB_2797834 | |
| HMB45 | * | Abcam | ab732 | HMB45;DT101;BC199 | AB_305844 | ||
| HO1 | Alexa Fluor 594 | * | Abcam | ab215098 | EPR1390Y | AB_2889222 | |
| HO1 | Alexa Fluor 488 | * | Abcam | ab214643 | EPR1390Y | AB_2889223 | |
| HP1 alpha | Alexa Fluor 647 | * | Abcam | ab198391 | EPR5777 | AB_2728810 | |
| HSF1 | * | StressMarq Biosciences | SMC-118 | 10H8 | AB_2120255 | ||
| Hsp90 beta (pS226) | * | Abcam | ab63562 | AB_1140737 | |||
| Hsp90b | * | Santa Cruz Biotechnology | sc-1057 | D-19 | AB_2121392 | Discontinued | |
| I-kappaB-Alpha | * | Cell Signaling Technology | 4814 | L35A5 | AB_390781 | ||
| IBA1 | FITC | * | Bioss | bs-1363R-FITC | AB_11098006 | ||
| IFNG | Alexa Fluor 488 | * | BioLegend | 502517 | 4S.B3 | AB_493030 | |
| IL-1 beta | FITC | * | BioLegend | 511705 | H1b-98 | AB_1236434 | |
| IL-3 | phycoerythrin | * | BD Biosciences | 554383 | Mp2-8F8 | AB_395358 | |
| IL-6 | FITC | * | BioLegend | 501103 | MQ2-13A5 | AB_315151 | |
| IRF-1 | Alexa Fluor 647 | * | Cell Signaling Technology | 14105 | D5E4 | AB_2798393 | |
| IRF-3 | * | Cell Signaling Technology | 10949 | D9J5Q | AB_2797733 | ||
| IRF-4 | * | Abcam | ab12501 | MUM1p | AB_2296152 | ||
| IRF-5 | Alexa Fluor 555 | * | Abcam | ab210656 | EPR17067 | AB_2889224 | |
| IRF-7 | phycoerythrin | * | Abcam | ab210435 | EPR4718 | Discontinued | |
| Lamin-A/C | Alexa Fluor 488 | * | Abcam | ab185014 | EPR4100 | AB_2889225 | |
| Lamin-B | Alexa Fluor 647 | * | Abcam | ab194108 | EPR8985(B) | AB_2889226 | |
| LAP | Alexa Fluor 488 | * | BioLegend | 349605 | TW4-2F8 | AB_10679043 | |
| LC3 | Alexa Fluor 647 | * | Cell Signaling Technology | 13394 | D3U4C | AB_2798205 | |
| LC3 | * | Abcam | ab81785 | AB_1640136 | |||
| MAD2L2 | Alexa Fluor 555 | * | Abcam | ab210653 | EPR13657 | AB_2889227 | |
| MDM2 | Alexa Fluor 647 | * | Abcam | ab216076 | SMP14 | AB_2889228 | |
| MERTK | * | Abcam | ab110108 | AB_10858972 | |||
| MGMT | FITC | * | EpiGentek | A52414-050 | AB_2889338 | ||
| MGMT | Alexa Fluor 647 | * | Millipore Sigma | MAB16200 AF647 | MT3.1 | AB_2281919 | |
| MGMT | Alexa Fluor 647 | * | Novus Biologicals | NB100-168 AF647 | MT 23.2 | AB_10002128 | |
| MGMT | * | Abcam | ab39253 | MT3.1 | AB_776338 | ||
| MGMT | * | Origene | TA800856GM | OTI4E6 | AB_2625715 | ||
| MITF | * | Abcam | ab12039 | C5 | AB_298801 | ||
| MITF | * | Abcam | ab80651 | AB_1603129 | |||
| MRP1 | Alexa Fluor 647 | * | BioLegend | 370203 | QCRL-2 | AB_2566664 | |
| mTOR (pS2448) | * | Cell Signaling Technology | 2971 | AB_330970 | |||
| mTOR (pS2448) | eFluor 660 | * | Thermo Fisher Scientific (eBioscience) | 50-9718-41 | MRRBY | AB_2574351 | |
| N-cadherin | * | Abcam | ab18203 | AB_444317 | |||
| Nestin | eFluor 570 | * | Thermo Fisher Scientific | 41-9843-80 | 10C2 | AB_2573652 | |
| Neurofibromin | * | Abcam | ab178323 | McNFn27b | AB_2728814 | ||
| NQO1 | Alexa Fluor 647 | * | Abcam | ab195874 | A180 | Discontinued | |
| NRF2 | Alexa Fluor 488 | * | Abcam | ab194984 | EP1808Y | AB_2889229 | |
| NTRK1 | * | Abcam | ab76291 | EP1058Y | AB_1524514 | ||
| Nuclear factor NF-kappa-B p65 subunit | Alexa Fluor 647 | * | Abcam | ab190589 | E379 | AB_2728800 | |
| Olig2 | Alexa Fluor 488 | * | Millipore Sigma | AB9610-AF488 | AB_570666 | ||
| Olig2 | Alexa Fluor 488 | * | Millipore Sigma | MABN50A4 | 211F1.1 | AB_11205039 | |
| Olig2 | * | Millipore Sigma | AB9610-AF555 | AB_570667 | |||
| p-P44/42 MAPK (Erk1: pY204; Erk2: pY187) | phycoerythrin | * | Cell Signaling Technology | 75165S | D1H6G | AB_2728835 | |
| p53 | Alexa Fluor 488 | * | Cell Signaling Technology | 5429S | 7F5 | AB_10695458 | |
| PARP-1 (D214-cleaved) | Alexa Fluor 555 | * | Cell Signaling Technology | 6894S | D64E10 | AB_10830735 | |
| PCNA | Alexa Fluor 647 | * | Abcam | ab201674 | PC10 | AB_2857977 | |
| PD-1 | Alexa Fluor 488 | * | Abcam | ab195885 | NAT105 | Discontinued | |
| PD-L1 | phycoerythrin | * | BioLegend | 329705 | 29E.2A3 | AB_940366 | |
| PLK1 | Alexa Fluor 647 | * | Bioss | bs-3535R-A647 | AB_2889334 | ||
| PML | Alexa Fluor 555 | * | Abcam | ab217524 | EPR16768 | Discontinued | |
| PMS2 | Alexa Fluor 555 | * | Abcam | ab203457 | EPR3947 | AB_2889230 | |
| POU5F1 | Alexa Fluor 555 | * | Abcam | ab210657 | EPR17929 | AB_2889231 | |
| PR | eFluor 660 | * | Thermo Fisher Scientific | 50-9764-80 | KMC912 | AB_2574363 | |
| PR | Alexa Fluor 594 | * | Abcam | ab207855 | YR85 | ||
| PTEN | * | Cell Signaling Technology | 9559 | 138G6 | AB_390810 | ||
| Rac | * | BD Biosciences | 610650 | 102/Rac1 | AB_397977 | ||
| RAD51 | Alexa Fluor 488 | * | Abcam | ab196449 | EPR4030(3) | AB_2889232 | |
| RB | phycoerythrin | * | Cell Signaling Technology | 14731 | 4H1 | AB_2798590 | |
| RFP | * | Thermo Fisher Scientific | R10367 | AB_10563941 | |||
| Runx | eFluor 660 | * | Thermo Fisher Scientific (eBioscience) | 50-9817-80 | R3-5G4 | AB_2574384 | |
| S100-A1 | * | Dako | Z0311 | AB_10013383 | |||
| S100-A9 | FITC | * | Abcam | ab7429 | MAC387 | AB_305923 | |
| SDHB | Alexa Fluor 647 | * | Abcam | ab197722 | 21A11AE7 | AB_2889233 | |
| SHMT2 | * | Abcam | ab224427 | AB_2889234 | |||
| SirT3 | * | Cell Signaling Technology | 2627 | C73E3 | AB_2188622 | ||
| SMAD2 | Alexa Fluor 647 | * | Abcam | ab196538 | EP567Y | AB_2889235 | |
| Smooth Muscle Myosin | Alexa Fluor 488 | * | Abcam | ab197524 | EPR5336(B) | AB_2889236 | |
| Sox2 | Alexa Fluor 647 | * | Abcam | ab192075 | AB_2728812 | ||
| Stat1 | phycoerythrin | * | Cell Signaling Technology | 80916 | D1K9Y | AB_2799965 | |
| Stat1 | Alexa Fluor 647 | * | Abcam | ab200417 | EPR4407 | AB_2889237 | |
| Stat4 | phycoerythrin | * | Abcam | ab216702 | EP1900Y | AB_2889238 | |
| Stat5a | Alexa Fluor 647 | * | Abcam | ab194309 | E289 | AB_2889239 | |
| Stat6 | Alexa Fluor 488 | * | Abcam | ab196478 | YE361 | AB_2889240 | |
| Tbr2 | * | Millipore Sigma | AB2283 | AB_10806889 | |||
| TCF1/TCF7 | Alexa Fluor 488 | * | Cell Signaling Technology | 6444 | C63D9 | AB_2797627 | |
| TDP43 | Alexa Fluor 488 | * | Abcam | ab193842 | EPR5810 | AB_2889241 | |
| TFEB | * | Abcam | ab2636 | AB_303224 | |||
| TIA-1 | * | Santa Cruz Biotechnology | sc-1751 | C-20 | AB_2201433 | Discontinued | |
| TIM3 | Alexa Fluor 647 | * | Cell Signaling Technology | 78226 | D5D5R | AB_2799911 | |
| TIM3 | Alexa Fluor 488 | * | Cell Signaling Technology | 54669 | D5D5R | AB_2799468 | |
| TLR3 | phycoerythrin | * | Thermo Fisher Scientific | MA5-16188 | 40C1285.6 | AB_2537707 | |
| TLR3 | * | Santa Cruz Biotechnology | sc-8691 | AB_2240700 | Discontinued | ||
| TLR3 | * | Santa Cruz Biotechnology | sc-8692 | C-20 | AB_793169 | ||
| TLR5 | phycoerythrin | * | Thermo Fisher Scientific | MA5-16241 | 19D759.2 | AB_2537759 | |
| TNF-alpha | Alexa Fluor 488 | * | BioLegend | 502917 | MAb11 | AB_493122 | |
| TNF-alpha | * | Abcam | ab11564 | MP6-XT3 | AB_298170 | ||
| TNF-R1 | * | Abcam | ab194814 | H398 | AB_2889242 | ||
| TP53 | Alexa Fluor 488 | * | Abcam | ab156030 | 9D3DE3 | AB_2889243 | |
| TSC2 (pS664) | * | Abcam | ab133465 | EPR8202 | AB_11157389 | ||
| TYRO3 | * | R&D Systems | MAB859-SP | 96201 | AB_2210975 | ||
| VDAC1 | Alexa Fluor 647 | * | Abcam | ab179840 | 20B12AF2 | ||
| VDAC1 | * | Abcam | ab15895 | AB_2214787 | |||
| XIAP | * | BD Biosciences | 610762 | 48/ hILP1/ XIAP | AB_398085 | ||
| AKT (pS473) | 0 | Cell Signaling Technology | 4051 | 587F11 | AB_331158 | ||
| AKT (pS473) | 0 | Cell Signaling Technology | 9271 | AB_329825 | |||
| AKT (pS473) | 0 | Cell Signaling Technology | 12694 | D9W9U | AB_2797994 | ||
| AKT (pT308) | phycoerythrin | 0 | Cell Signaling Technology | 13842 | D25E6 | AB_2798327 | |
| AKT (pT308) | 0 | Cell Signaling Technology | 2965 | C31E5E | AB_10233973 | ||
| AKT (pT308) | 0 | Cell Signaling Technology | 5106 | L32A4 | AB_836861 | ||
| AKT (pT308) (pan) | 0 | Abcam | ab8933 | AB_306868 | |||
| Akt-1 (pS473) | 0 | Cell Signaling Technology | 4060 | D9E | AB_2315049 | ||
| AKT1 (pS473) | Alexa Fluor 647 | 0 | Abcam | ab194200 | EP2109Y | ||
| AKT1 (pT308) | 0 | Abcam | ab105731 | 18F3.H11 | AB_10900490 | ||
| ALDH1A1 | phycoerythrin | 0 | Cell Signaling Technology | 65583 | D9Q8E | AB_2799687 | |
| ALK | 0 | Santa Cruz Biotechnology | sc-53157 | ALK1 | AB_628703 | ||
| Amyloid Precursor Protein | Alexa Fluor 647 | 0 | Abcam | ab199549 | Y188 | AB_2868404 | |
| Amyloid-beta precursor protein | Alexa Fluor 488 | 0 | BioLegend | 803013 | 6E10 | AB_2564765 | |
| Aquaporin 4 | 0 | Abcam | ab9512 | 4/18 | AB_307299 | ||
| AXL | 0 | Cell Signaling Technology | 8661 | C89E7 | AB_11217435 | ||
| BIM | Alexa Fluor 488 | 0 | Abcam | ab200667 | Y36 | AB_2868516 | |
| BRD4 | Alexa Fluor 488 | 0 | Abcam | ab197606 | EPR5150(2) | AB_2868517 | |
| c-Jun | FITC | 0 | Santa Cruz Biotechnology | sc-1694 FITC | AB_631263 | Discontinued | |
| c-Jun (pS63) | 0 | Santa Cruz Biotechnology | sc-822 | KM-1 | AB_627262 | ||
| c-Myc | 0 | Santa Cruz Biotechnology | sc-40 TRITC | 9E10 | AB_627268 | ||
| CD103 | APC | 0 | Thermo Fisher Scientific | 17-1038-41 | B-Ly7 | AB_10669816 | |
| CD11a | FITC | 0 | Thermo Fisher Scientific | 11-0119-41 | HI111 | AB_10597888 | |
| CD11c | phycoerythrin | 0 | BioLegend | 337205 | Bu15 | AB_1236439 | |
| CD11c | APC | 0 | BioLegend | 337207 | Bu15 | AB_1279068 | |
| CD11c | 0 | BioLegend | 117331 | N418 | AB_10900261 | ||
| CD123 | 0 | R&D Systems | MAB301-100 | 32703 | AB_2124263 | ||
| CD13 | 0 | BD Biosciences | 561361 | WM15 | AB_10645787 | ||
| CD133 | phycoerythrin | 0 | Thermo Fisher Scientific | 12-1338-41 | TMP4 | AB_1582258 | |
| CD137 | 0 | Cell Signaling Technology | 34594 | D2Z4Y | AB_2747410 | ||
| CD141 | Alexa Fluor 488 | 0 | Abcam | ab237063 | EPR4051 | AB_2889244 | |
| CD152 | phycoerythrin | 0 | BioLegend | 369603 | BNI3 | AB_2566796 | |
| CD163 | phycoerythrin | 0 | Abcam | ab95613 | GHI/61 | AB_10681084 | |
| CD163 | Alexa Fluor 647 | 0 | BioLegend | 326507 | RM3/1 | AB_893268 | |
| CD163 | Alexa Fluor 647 | 0 | BioLegend | 333619 | GHI/61 | AB_2563474 | |
| CD1b | phycoerythrin | 0 | Abcam | ab232916 | SN13 | AB_2889245 | |
| CD1d | 0 | Abcam | ab11076 | NOR3.2/13.17 | AB_297728 | ||
| CD2 | FITC | 0 | BioLegend | 300206 | RPA-2.10 | AB_314030 | |
| CD20 | FITC | 0 | BioLegend | 302303 | 2H7 | AB_314251 | |
| CD20 | Alexa Fluor 488 | 0 | Thermo Fisher Scientific (eBioscience) | 53-0202-80 | L26 | AB_10734357 | |
| CD206 | Alexa Fluor 647 | 0 | BioLegend | 321116 | 15-2 | AB_571881 | |
| CD26 | Alexa Fluor 647 | 0 | Santa Cruz Biotechnology | sc-52642 AF647 | 5E8 | AB_629016 | |
| CD28 | Alexa Fluor 488 | 0 | Santa Cruz Biotechnology | sc-19655 AF488 | CD28.2 | AB_627002 | |
| CD28 | 0 | Abcam | ab113358 | AB_10861658 | |||
| CD31 | ATTO 565 | 0 | StressMarq Biosciences | SPC-1283D-A565 | AB_2712902 | ||
| CD33 | FITC | 0 | BD Biosciences | 340533 | P67.6 | AB_400047 | |
| CD33 | Alexa Fluor 647 | 0 | Santa Cruz Biotechnology | sc-53199 AF647 | 6C5/2 | AB_782152 | |
| CD35 | phycoerythrin | 0 | BioLegend | 333405 | E11 | AB_2085021 | |
| CD35 | phycoerythrin | 0 | Abcam | ab82439 | E11 | AB_1658461 | |
| CD35 | FITC | 0 | Abcam | ab82441 | E11 | AB_2085008 | |
| CD365 | phycoerythrin | 0 | BioLegend | 353903 | 1D12 | AB_11125165 | |
| CD3d | FITC | 0 | Abcam | ab34722 | CD3-12 | AB_2889246 | |
| CD3e | eFluor 660 | 0 | Thermo Fisher Scientific | 50-0037-41 | OKT3 | AB_2574150 | |
| CD3e | APC | 0 | Thermo Fisher Scientific | 17-0038-41 | UCHT1 | AB_10804761 | |
| CD3e | Alexa Fluor 647 | 0 | BioLegend | 300422 | UCHT1 | AB_493092 | |
| CD45R | eFluor 660 | 0 | Thermo Fisher Scientific (eBioscience) | 50-0452-82 | RA3-6B2 | AB_11217671 | |
| CD45R | eFluor 570 | 0 | Thermo Fisher Scientific (eBioscience) | 41-0452-80 | RA3-6B2 | AB_2573598 | |
| CD45R | Alexa Fluor 647 | 0 | BioLegend | 103229 | RA3-6B2 | AB_492875 | |
| CD56 | FITC | 0 | Thermo Fisher Scientific | 11-0566-41 | TULY56 | AB_2572458 | |
| CD56 | eFluor 660 | 0 | Thermo Fisher Scientific | 50-0565-80 | 5tukon56 | AB_2574160 | |
| CD56 | Alexa Fluor 647 | 0 | BioLegend | 362513 | 5.1H11 | AB_2564086 | |
| CD56 | Alexa Fluor 488 | 0 | Abcam | ab200333 | EPR2566 | AB_2728815 | |
| CD68 | eFluor 570 | 0 | Thermo Fisher Scientific | 41-0687-80 | 815CU17 | AB_2573600 | |
| CD68 | ATTO 655 | 0 | StressMarq Biosciences | SMC-257 | 6F3 | AB_2699772 | |
| CD68 | Alexa Fluor 488 | 0 | BioLegend | 137011 | FA-11 | AB_2074847 | |
| CD69 | 0 | Abcam | ab202909 | AB_2889247 | |||
| CD83 | 0 | BioLegend | 305301 | HB15e | AB_314509 | ||
| CD85j | Alexa Fluor 647 | 0 | BioLegend | 333709 | GHI/75 | AB_2136384 | |
| CD8a | Alexa Fluor 488 | 0 | BioLegend | 301024 | RPA-T8 | AB_2561282 | |
| CEP170 | 0 | Abcam | ab72505 | AB_1268101 | |||
| Cleaved PARP (D214) | Alexa Fluor 647 | 0 | BD Biosciences | 558710 | F21-852 | AB_1645431 | |
| CtlP | Alexa Fluor 647 | 0 | Novus Biologicals | NBP1-41205AF647 | 19E8 | AB_2238010 | |
| CXCR5 | FITC | 0 | BioLegend | 356913 | J252D4 | AB_2561895 | |
| CXCR5 | 0 | GeneTex | GTX100351 | AB_1240668 | |||
| CXCR5 | 0 | R&D Systems | MAB190-SP | 51505 | AB_2292654 | ||
| DNMT3A | 0 | Abcam | ab13888 | 64B1146 | AB_300714 | ||
| E-cadherin | FITC | 0 | BioLegend | 324103 | 67A4 | AB_756065 | |
| ERK | Alexa Fluor 488 | 0 | Cell Signaling Technology | 4780 | 137F5 | AB_1903964 | |
| ERK (pT202; pY204) | Alexa Fluor 647 | 0 | BioLegend | 369503 | 6B8B69 | AB_2571895 | |
| FGFR1 | phycoerythrin | 0 | Cell Signaling Technology | 12777 | D8E4 | AB_2798023 | |
| FOXO3 | Alexa Fluor 647 | 0 | Abcam | ab196539 | EP1949Y | AB_2728818 | |
| FOXO3a | 0 | Cell Signaling Technology | 2497S | 75D8 | AB_836876 | ||
| FOXP3 | Alexa Fluor 488 | 0 | BioLegend | 320011 | 150D | AB_439747 | |
| GATA3 | Alexa Fluor 594 | 0 | BioLegend | 653816 | 16E10A23 | AB_2563353 | |
| GFAP | 0 | Millipore Sigma | MAB3402C3 | AB_11213580 | |||
| Granzyme A | eFluor 660 | 0 | Thermo Fisher Scientific | 50-9177-41 | CB9 | AB_2574330 | |
| Granzyme B | FITC | 0 | Abcam | ab17929 | B18.1 | AB_444109 | |
| Granzyme B | Alexa Fluor 647 | 0 | BioLegend | 515405 | GB11 | AB_2294995 | |
| Granzyme B | 0 | Millipore Sigma | HPA003418 | AB_1079020 | |||
| Granzyme B | 0 | Santa Cruz Biotechnology | sc-73620 | GRB7 | AB_2114695 | ||
| GRHL2 | 0 | Abcam | ab211360 | AB_2889248 | |||
| HIF1-alpha | Alexa Fluor 647 | 0 | BioLegend | 359705 | 546-16 | AB_2563331 | |
| Histone H2A.X (pS139) | 0 | Cell Signaling Technology | 2577S | Ser 139 | AB_2118011 | ||
| Homeo Box C10 | 0 | Abcam | ab153904 | AB_2687827 | |||
| HSF1 | FITC | 0 | Abcam | ab186360 | 4B4 | Discontinued | |
| HSF1 | ATTO 655 | 0 | StressMarq Biosciences | SMC-477D A655 | 4B4 | AB_2702303 | |
| HSF1 | ATTO 565 | 0 | StressMarq Biosciences | SMC-476D-A565 | 10H4 | AB_2702282 | |
| HSF1 | ATTO 488 | 0 | StressMarq Biosciences | SMC-118D-A488 | 10H8 | AB_2698025 | |
| IDO | phycoerythrin | 0 | Cell Signaling Technology | 10312 | D5J4E | AB_2797718 | |
| IDO | eFluor 660 | 0 | Thermo Fisher Scientific | 50-9750-80 | V1NC3IDO | AB_2574357 | |
| IDO | 0 | Millipore Sigma | MAB10009 | 1F8.2 | AB_1977068 | ||
| LAG-3 | phycoerythrin | 0 | BD Biosciences | 565617 | T47-530 | AB_2889327 | |
| LAG-3 | phycoerythrin | 0 | R&D Systems | FAB2319P | AB_2133351 | ||
| LAG-3 | FITC | 0 | Thermo Fisher Scientific | 11-2239-42 | 3DS223H | AB_2572486 | |
| LAG-3 | FITC | 0 | Adipogen | AG-20B-0012F-C100 | 17B4 | AB_2490196 | |
| LAG-3 | 0 | Abcam | ab40465 | 11E3 | AB_776102 | ||
| lkBa (pS32; pS36) | Alexa Fluor 647 | 0 | Thermo Fisher Scientific (eBioscience) | 50-9035-41 | RILYB3R | AB_2574311 | |
| Ly6G | 0 | BioLegend | 127629 | 1A8 | AB_10899738 | ||
| MCL1 | Alexa Fluor 647 | 0 | Abcam | ab197035 | Y37 | AB_2800505 | |
| MCP1 | FITC | 0 | LifeSpan BioSciences | LS-C211505 | AB_2889339 | ||
| MCP1 | Alexa Fluor 647 | 0 | BD Biosciences | 563496 | 5D3-F7 | AB_2738242 | |
| MGMT | 0 | Cell Signaling Technology | 2739 | AB_2297658 | |||
| MGMT | 0 | Origene | TA800732EM | OTI1B12 | AB_2625647 | ||
| MIP3 Alpha | 0 | Abcam | ab122889 | AB_10903028 | |||
| MITF | Alexa Fluor 488 | 0 | Novus Biologicals | NB100-56561AF488 | 21D1418 | AB_838580 | |
| Mus81 | Alexa Fluor 488 | 0 | Novus Biologicals | NB100-2064 AF488 | MTA30 2G10/3 | AB_1109412 | |
| NANOG | Alexa Fluor 647 | 0 | Abcam | ab195018 | EPR207(2) | AB_2889249 | |
| NeuN | Alexa Fluor 555 | 0 | Abcam | ab207281 | EPR12763 | AB_2889250 | |
| NeuN | Alexa Fluor 488 | 0 | Millipore Sigma | MAB377X | A60 | AB_2149209 | |
| Neurofilament L | Alexa Fluor 488 | 0 | Cell Signaling Technology | 8024 | C28E10 | AB_10860421 | |
| NGF receptor | FITC | 0 | BioLegend | 345103 | ME20.4 | AB_1937226 | |
| none (Nitrotyrosine) | FITC | 0 | Abcam | ab183390 | 39B6 | AB_2889251 | |
| NOS2 | Alexa Fluor 647 | 0 | Cell Signaling Technology | 48866 | D6B6S | AB_2799345 | |
| NOS2 | Alexa Fluor 555 | 0 | Abcam | ab209594 | EPR16635 | AB_2889252 | |
| Notch1 | 0 | Abcam | ab8925 | AB_306863 | |||
| NRARP | Alexa Fluor 555 | 0 | Bioss | bs-11914R-A555 | AB_2889335 | ||
| p44/42 (pT202; pY202; pY204) | Alexa Fluor 488 | 0 | Cell Signaling Technology | 4374S | E10 | AB_10705598 | |
| p53 | 0 | Santa Cruz Biotechnology | sc-126 | DO-1 | AB_628082 | ||
| p63 | 0 | BioCare | PM 366 AAK, HK | 4A4 | AB_2889329 | ||
| PD-1 | phycoerythrin | 0 | BioLegend | 329905 | EH12.2H7 | AB_940481 | |
| PD-1 | Alexa Fluor 488 | 0 | BioLegend | 329935 | EH12.2H7 | AB_2563593 | |
| PD-1 | Alexa Fluor 488 | 0 | BioLegend | 367407 | NAT105 | AB_2566677 | |
| PD-1 | 0 | Thermo Fisher Scientific | 14-9985-81 | J43 | AB_468663 | ||
| PD-1 | 0 | Abcam | ab63477 | RMP1-14 | AB_2159165 | ||
| PD-1 | 0 | R&D Systems | AF1021 | AB_354541 | |||
| PD-L1 | 0 | Dako | M365329-1 | 22C3 | |||
| PDGFR | Alexa Fluor 555 | 0 | Abcam | ab206874 | Y92 | AB_2728816 | Discontinued |
| PDGFR-alpha | Alexa Fluor 555 | 0 | Cell Signaling Technology | 8893S | D13C6 | AB_2797679 | |
| POU5F1 | Alexa Fluor 555 | 0 | Cell Signaling Technology | 4439 | C30A3 | AB_10922586 | |
| PrlR | Alexa Fluor 488 | 0 | Abcam | ab201760 | EPR7184(2) | AB_2889253 | |
| RAD51 | 0 | Thermo Fisher Scientific (Invitrogen) | MA5-14419 | 51RAD01 (3C10) | AB_10985045 | ||
| Runx2 | Alexa Fluor 647 | 0 | Abcam | ab215955 | EPR14334 | AB_2889254 | |
| S100-A4 | FITC | 0 | BioLegend | 370007 | NJ-4F3-D1 | AB_2572073 | |
| S100-A4 | Alexa Fluor 488 | 0 | BioLegend | 370007 | NJ-4F3-D1 | AB_2572073 | |
| SATB1 | Alexa Fluor 647 | 0 | Abcam | ab204469 | EPR3895 | AB_204469 | |
| SEC61A | Alexa Fluor 647 | 0 | Abcam | ab205795 | EPR14379 | Discontinued | |
| Sox2 | Alexa Fluor 488 | 0 | BioLegend | 656109 | 14A6A34 | AB_2563956 | |
| SPI1 | Alexa Fluor 647 | 0 | Cell Signaling Technology | 2240 | 9G7 | AB_2186911 | |
| SSTR2 | 0 | Abcam | ab216649 | UMB1 | AB_2889255 | ||
| Stat6 | Alexa Fluor 555 | 0 | Abcam | ab207014 | YE361 | AB_2889256 | |
| TP53 | Alexa Fluor 488 | 0 | Abcam | ab204245 | DO-1 | Discontinued | |
| TPCN2 | 0 | Abcam | ab119915 | AB_10903692 | |||
| YAP1 | Alexa Fluor 647 | 0 | Cell Signaling Technology | 38707 | D8H1X | AB_2728837 |
